Single protein production (SPP) system in Escherichia coli

Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli

Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to produce specific antibody by egg yolk antibody (IgY...

Advances and challenges in recombinant protein expression in Escherichia coli

Production of MAG1 Antigen of Toxoplasma gondii in Escherichia coli

  Introduction : Toxoplasmosis is a parasitic infection caused by the protozoan Toxoplasma gondii it leads to serious medical problems in congenitally-infected and immunocompromised individuals, while it is quite harmless in immunocompetent individuals. Toxoplasma tissue cyst matrix protein (MAG1) induces early humoral and cell-mediated immune responses. Previous studies suggested recombinant M...

Establishment of molybdeum cofactor detection system in Escherichia coli

In the current study, in order to verify the presence of bacterial Molybdenum cofactor, an indirect approach was made by showing the activity of BisC enzyme as a reporter gene. The activity of the BisC enzyme is dependent to Bis-MGD cofactor. BisC enzyme converts biotin sulfoxide to biotin under abiotic stress condition and it can also reduce TMANO and because of this property it was applied on...

Improvement in recombinant protein production in ppGpp-deficient Escherichia coli.

Maintaining a metabolically productive state for recombinant Escherichia coli remains a central problem for a wide variety of growth-dependent biosynthesis. This problem becomes particularly acute under conditions of minimal cell growth such as fed-batch fermentations. In this, we investigated the possibility of manipulating the protein synthesis machinery of E. coli whereby synthesis of foreig...